A Robust Visible Spectrophotometric Method for The Determination of Selected Pharmaceutical Formulations Based on Cerium(Iv)-Mediated Oxidation and Methylene Blue Bleaching
Author(s): Rajitha Balusan
Publication #: 2606053
Date of Publication: 18.11.2025
Country: India
Pages: 1-12
Published In: Volume 11 Issue 6 November-2025
DOI: https://doi.org/10.62970/IJIRCT.v11.i6.2606053
Abstract
A simple, sensitive, and reproducible spectrophotometric method was developed for the quantitative estimation of Cetirizine Dihydrochloride (CDC), Imatinib Mesylate (INM), and Voriconazole (VNZ) in both pure drug substances and pharmaceutical dosage forms. The analytical procedure is based on the oxidation of the selected drugs by a measured excess of Cerium(IV) in an acidic medium. The residual Cerium(IV) was subsequently determined through its reaction with Methylene Blue, resulting in a measurable decrease in dye absorbance.
The proposed method was systematically validated according to established analytical guidelines. Key performance characteristics, including linearity, accuracy, precision, sensitivity, robustness, and ruggedness, were evaluated. The method demonstrated excellent analytical performance, providing percentage recoveries in the range of 97–100% with relative standard deviation values below 2%, indicating satisfactory accuracy and precision. Detection and quantification limits confirmed the high sensitivity of the procedure. Furthermore, statistical comparison of the obtained results with those of established reference methods using Student’s t-test and F-test revealed no significant differences in accuracy or precision.
Owing to its simplicity, cost-effectiveness, and reliable analytical performance, the developed method is well suited for routine quality-control analysis of these pharmaceutical compounds in bulk and tablet formulations.
Keywords: Cetirizine Dihydrochloride, Imatinib Mesylate, Voriconazole, Spectrophotometric Analysis, Cerium(IV), Methylene Blue, Pharmaceutical Quality Control, Method Validation.
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